Isolation of Epstein - Barr virus genomes using pulse-field gel electrophoresis
نویسندگان
چکیده
منابع مشابه
Replication of latent Epstein-Barr virus genomes in Raji cells.
The replication of the 50 to 60 latent, predominantly extrachromosomal, Epstein-Barr virus genomes maintained by the Burkitt-lymphoma-derived Raji cell line was investigated by using a Meselson-Stahl density transfer approach. Samples of DNA isolated from cells cultivated for different periods in bromodeoxyuridine-supplemented medium were fractionated according to density, and the distribution ...
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Introduction: Epstein – Barr virus (EBV) is a herpesvirus which establishes a persistent life-long infection in over 95% of adults world wide. Infection is usually asymptomatic but the virus is associated with a variety of disease, including nasopharyngeal carcinoma (NPC), Burkitt’s lymphoma (BL), Multiple sclerosis MS, EBV-associated B-cell lymphoproliferative disease (BLPD), Hodgkin and non...
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The technique of pulse-field gel electrophoresis (PFGE) makes use of restriction enzymes that cleave DNA at specific restriction sites, generating DNA fragments of different sizes. These fragments can be separated according to size by agarose-gel electrophoresis and visualized using DNA stains (e.g. ethidium bromide), giving a characteristic banding pattern. Given the high specificity of restri...
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Background & Objective: Ovarian cancer is one of the most common cancers amongst women. The association of Human papillomavirus (HPV) and Epstein-Barr virus (EBV) with ovarian cancer is inconclusive; therefore, the aims of this study were to evaluate the frequency of HPV and EBV in malignant, ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.11.2901